Credentials will be securely shared with you via Globus upon completion of your sequencing and analysis.
#Sequencher uw download#
You will be able to download these files using Globus. Raw sequence data for your samples will be stored in PacBio's bam file format.
#Sequencher uw how to#
Await a sequencing report that will include details regarding how to download your sequence data.Await email confirmation from us that your samples have arrived.Wait for an estimate of cost form to be issued via email, sign it, and return to us.Email the completed form to us at Prepare your samples and optionally prepare SMRTbell libraries for sequencing.For external requests, we accept money order, VISA, and MasterCard payments. , selecting DNA or Iso-Seq service, and include a purchase order (PO) number, fund code, or budget number. If you are unsure which services you require, contact us for a consultation and a quote.Please indicate your organism of interest, genome size or amplicon length, and desired coverage if known.Iso-Seq whole transcriptome (3 pooled tissues targeting 500k reads per tissue) Services Microbial Genome Analysis in SMRTLink v11Īmplicon Sequencing (customer-barcoded pooled amplicons 1-5 kb) Services Multiplex Microbe (16 pooled samples, genomic DNA + plasmids) Services Project Examples HiFi Data collection (25-30X coverage of a human-sized genome) Services *If you would like us to demultiplex your custom barcodes, please specify this on the request form and include your barcode sequences. Standard analysis with default parameters through cluster and polishing.)
SMRT Link 11 Iso-Seq Analysis Pipeline CCS service fee applies (symmetric or asymmetric barcodes, standard PacBio barcode sequence sets ) May include CpG methylation calls ( primrose) upon request.) (Circular consensus sequence generation with loose or stringent quality filters. Assembly will use HiFi reads and include base modification analysis.)ĬCS service fee applies to long movies, included with short movies (One attempt at genome assembly using default parameters. SMRT Link 11 Microbial Genome Analysis pipeline We include the following analysis services for specific sequencing project types:īacterial genomic DNA and other small genomes <5.5Mbp We can also generate filtered subread sequences in FASTA or FASTQ format upon request. **Host institution and HHMI investigators have priority for the sequencing queue.įor Sequel II SMRT cells, we provide the entire raw data folder as a tar.gz file, including the main raw data file in the subreads.bam format. *Note: Prices effective and subject to change without notice. Sequel II CCS Data Analysis (per long movie)
#Sequencher uw movie#
Sequel II SMRT Cell 8M with 30-hour movie (long)Īgilent Femto Pulse (per run up to 11 samples)Īgilent Bioanalyzer (per run up to 11 samples) Sequel II SMRT Cell 8M with 15-hour movie (short) Sample QC (per DNA sample/submitted library) *Maximum number of genomes per pool is dependent on expected genome size and desired coverage level. This service includes cDNA generation from total RNA and SMRTbell addition. Transcriptome and Isoform sequencing with PacBio’s Iso-Seq protocol. High Fidelity (HiFi) Template Preparation (15-22kb)
We provide library preparations and sequencing for: Services and Rates We are now offering sequencing only on the Sequel II platform.
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